Human aldolase B cDNA detects a Pvu II RELP in healthy indiviuals

Human aldolase B cDNA detects a Pvu II RFLP in healthy individuals

CODING SEQUENCE: Human aldolase B gene (ALDOB). The gene is a single copy and its chromosomal location is 9q21.3-q32 MENDELIAN INHERITANCE: Demonstrated in one family. The mother and the son are heterozygotes, whereas the father is a homozygote. OTHER COMMENTS: Hereditary fructose intolerance is due to a lack of aldolase B activity in liver. Although we observed no 2.3 kb homozygotes, no sign o...

Complete amino acid sequence for human aldolase B derived from cDNA and genomic clones.

Several aldolase B clones from a human liver cDNA library have been identified by using a rabbit aldolase A cDNA as a hybridization probe. The most complete of these, pHL413, is 1389 base pairs long and covers approximately equal to 80% of the length of the mRNA, including 90% of the translated region. The cDNA, pHL413, was used to identify a genomic clone, lambda HG313, which encoded the remai...

Human cDNA probe (OL167,D21S109) detects RFLP on chromosome 21.

Aldolase from Maize Derived from cDNA

A cDNA library was synthesized from maize anaerobic root mRNA and screened with cDNA specific to the anaerobically induced Zea mays cytoplasmic aldolase. At least 1% of the cDNA of the library corresponded to maize cytoplasmic aldolase. The sequence of four overlapping cDNA clones encoded a protein of molecular weight 38,611 homologous to aldolase. These cDNAs were polymorphic at three bases an...

A frequent Pvu II RFLP of the human gastrin releasing peptide gene

SOURCE/DESCRIPTION: pB12, a 650 bp cDNA cloned at the Pst I and Hind III sites of pSP64 (Spindel et. al.). P0LYM0RPHIS: Pvu II detects two allelic fragments at 8.9 kb and 8.3 kb. OTHER COMMENTS: To obtain better separation of bands electrophorese on a 0.8% agarose gel until the xylene cyanol dye is at least 9 cm from the origin. ACKNOWLEDGEMENTS: We thank Ms. Denise Boorman for technical help.